Figure 5.

Effects of AK144841 silencing on the mSCC-38 cell transcriptome. mSCC-38 cells were transfected with two distinct siRNAs directed against AK144841 (Si1AK and Si2AK) and a control siRNA (SiC). The gene expression profiles were determined at 48 h post-transfection using a pan genomic microarrays (gene expression 8 × 60,000 microarrays from Agilent). The graph shows the heat map representing the normalized log₂ intensity of the 129 genes whose expression is statistically modulated by AK144841 siRNA in the different experimental conditions. Threshold values used to select the 129 up- and down-regulated genes represented on the heat map are average expression >7.0, absolute log FC >0.6, and adjusted p value <0.05. NT1–4, four samples of non-transfected mSCC-38 cells. SiC1–3, three samples of mSCC-38 cells transfected with a non-relevant siRNA (SiC). Si1AK1–4, four samples of mSCC-38 cells transfected with Si1AK, the first AK144841-specific siRNA. Si2AK1–3, three samples of mSCC-38 cells transfected with Si2AK, the second AK144841 siRNA.