Figure 6.

Effect of AK144841 overexpression on genes whose expression was up-regulated upon AK144841 silencing. mSCC-38 cells were transfected with plasmids pLJM1-AKL and -AKS or an empty pLJM1 using Lipofectamine 3000 and harvested after 72 h. Total RNA was extracted, and the expression of Cgref1, Brsk1, Basp1, Dusp5, Btg2, Anpep, Dhrs9, Stfa2, Tpm1, SerpinB2, Cpa4, Crct1, Cryab, Il24, Csf2, and Rgs16 mRNA was measured by qPCR using specific primers. The histograms represent the expression of each gene normalized to their expression in cells transfected with the empty pLJM1 (Mock). Error bars represent the S.E. of five individual experiments, and asterisks represent the Student's t test p value (*, p < 0.05; **, p < 0.01; ***, p < 0.001). The transfection efficiency estimated using a pLJM1-GFP vector was ∼30%. The primer sequences can be obtained by request.