Figure 10.

STUB1 selectively inhibited the growth of RUNX1–RUNX1T1 leukemia cells. A, a vector control or STUB1 (coexpressing GFP) was transduced into K562, THP1, HEL, and HL-60 cells. The mixed transduction culture containing both transduced GFP⁺ and nontransduced GFP⁻ cells were passaged to score the frequency of the GFP⁺ cell by flow cytometric analysis as a measure of the impact of the transduced gene on cellular proliferation rate. The initial frequency of GFP⁺ cells immediately after transduction was set as 1. Two independent experiments were performed, and data are shown as mean ± S.E. STUB1 overexpression showed only a marginal effect in these non-RUNX1–RUNX1T1 myeloid leukemia cells. B, human CB cells and those expressing RUNX1–RUNX1T1 were transduced with a vector control or STUB1 (coexpressing GFP). The cells were cultured in cytokine containing media to monitor the changes of GFP frequency. STUB1 inhibited the growth of RUNX1–RUNX1T1-expressing CB cells, but not that of normal CB cells.