Figure 7.

STUB1 depletion increased stability of RUNX1–RUNX1T1. A, Kasumi-1 cells were transduced with a vector control or two independent gRNAs targeting STUB1. The gRNAs showed efficient depletion of STUB1, which resulted in the increased expression of RUNX1 and RUNX1–RUNX1T1. B, Kasumi-1 cells transduced with a vector or STUB1 gRNAs were treated with 25 μg/ml of CHX for the indicated times, and cell extracts were analyzed with anti-RUNX1 antibody. Band intensities of RUNX1 and GAPDH were quantified with Multi Gauge (Science Lab), and the intensities of RUNX1–RUNX1T1 relative to GAPDH are shown. The value of RUNX1–RUNX1T1/GAPDH without CHX treatment was set to 1. Representative data (upper) and cumulative data from three independent experiments (lower, mean ± S.E., *, p < 0.05; +, p < 0.05) are shown. IB, immunoblot.