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. 2017 Jun 2;292(30):12560–12576. doi: 10.1074/jbc.M117.791350

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — WT and mutant PR-A and PR-B isoforms are expressed at equivalent levels in stably transfected hTERT-HM cells. A, hTERT immortalized human myometrial cells were stably transformed using recombinant lentiviruses expressing PR-B_WT, PR-B_mDBD, PR-A_WT, and PR-A_mDBD conjugated to GFP at their N-terminal ends. PR-B DBD and PR-A DBD mutants (PR_mDBD) contained a triple mutation (G584E/S585G/V589A) within the P-box region of the DBD. B, immunoblotting of total cell lysates revealed that levels of PR expression in the four stably transfected cell lines were equivalent. LBD, ligand-binding domain.