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. 2017 Jun 6;292(30):12606–12620. doi: 10.1074/jbc.M117.789008

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — Sequencing of the heterogeneous disaccharide released from RAX by differential labeling of the reducing end. A, GC-MS chromatograms of the RAX product. The background reaction buffer is shown in 1. The disaccharide was reduced before (2) and after (3) acid hydrolysis, and then acetylated. Only Ara is visible in the non-hydrolyzed sample (the missing Gal peak is indicated with a star); whereas, both Ara and Gal are visible when the monosaccharides are generated before reduction and acetylation. Monosaccharide standards are shown in 4 and are labeled. B, FACE analysis of the ANTS-labeled disaccharide. The NF2523 product was labeled and then acid hydrolyzed. The products were compared with various standards. Lane 1 = ANTS; lane 2 = ANTS-labeled product hydrolysate; lane 3 = ANTS-labeled Ara; lane 4 = ANTS-labeled Gal.