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. 2017 Jun 5;292(30):12691–12701. doi: 10.1074/jbc.M116.769695

Figure 1.

Figure 1.

Overexpression of NLRC3 inhibits NALP3-induced IL-1β secretion. A and B, IL-1β secretion in ectopically assembled NALP3 inflammasomes in HEK293FT cells transfected with 500 ng of pro-IL-1β, 250 ng of ASC, and 250 ng of pro-caspase-1 plasmids and the indicated amount of NALP3 (A) or 1 μg of NALP3 and the indicated amount of NLRC3 (B). Each condition was duplicated. Secreted IL-1β was quantified 24 h after transfection in cell-free supernatants by ELISA. C, IL-1β cleavage in HEK293FT cells with ectopically assembled NALP3 inflammasomes. NT, non-transfected. D, NLRC3 mRNA levels in the THP-1 OvCont and OvNLRC3 cell lines. Quantitative PCR results of relative mRNA of NLRC3/β-actin are given. The NLRC3/β-actin ratio of OvCont cells was assigned as 1 arbitrary unit. E, NLRC3 protein levels in THP-1 OvControl and OvNLRC3 stable lines. F and G, IL-1β secretion in THP-1 OvNLRC3 and THP-1 OvControl stable lines in response to nigericin (F) and MSU (G) treatment.