Figure 2.

Knockdown of NLRC3 in THP-1 cells results in an increase in IL-1β secretion and pro-IL-1β and pro-caspase-1 cleavage. A, NLRC3 protein levels in three different stable THP-1 NLRC3 KD cell lines (shD6, shD8, and shD9) and their control line (shcont). B, IL-1β secretion in shD6, shD8, shD9, and shcont THP-1 stable cell lines in response to MSU stimulation (representative result of four independent sets of experiments). NS, not significant. C and D, IL-1β secretion in shNLRC3 THP-1 cell lines in response to ATP (C) (representative result of three independent sets of experiments) and nigericin (D) (combined results of two independent sets of experiments) stimulation. E, pro-IL-1β cleavage in the cell lysate and supernatant of the shcont and shNLRC3 cell lines in response to nigericin stimulation. Control (C) samples represent cells not treated with nigericin. Two independent samples were treated with nigericin (N1 and N2). Exp, exposure. F, pro-caspase-1 cleavage in the supernatant (Sup) of nigericin-treated shcont and shNLRC3 cells. G, LDH results of nigericin-treated shcontrol and shNLRC3 THP-1 cells. Shown are representative result of three sets of independent experiment. H, IL-1β secretion in response to P. aeruginosa infection of the shcont and shNLRC3 cell lines. Two-tailed Student's t test was performed.