Figure 1.

siRNA-based screening reveals a specific role for SMYD2 in BMP2-induced target gene expression. A, knockdown of SMYD family KMTs by siRNAs did not affect TGFβ1-induced target gene activation. HaCaT cells were transfected with the siRNAs against SMYD1 to SMYD5 as indicated. Two days later, cells were starved overnight with non-serum medium and then treated with 5 ng/ml TGFβ1 for 4 h. The cells were harvested for preparation of total RNAs and subsequent qRT-PCR analysis of PAI1, p21, and SMAD7 transcripts. B, knockdown of SMYD2 but not other SMYDs specifically impairs BMP2-induced target gene expression. HaCaT cells were treated as above except that TGFβ1 was replaced with 25 ng/ml BMP2, and qRT-PCR analysis was performed for BMP target genes ID1, ID2, and SMAD6.