Table 1.

Antibody/F_ab binding analysis using ITC and SPR

Clone	Aβ peptide	Kon in 1/Ms	koff in 1/s	KD
				SPR	ITC
				nm	nm
6	Human pE3–18 pEFRHDSGYEVHHQKLV	6.89 × 105	4.58 × 10−3	6.7	4.85 ± 0.35
24		2.41 × 105	0.42 × 10−3	1.8	6.85 ± 1.56
17		25.0 × 105	3.30 × 10−3	1.3	0.99 ± 0.31
6	Human 1–18 DAEFRHDSGYEVHHQKLV	184	>5.70 × 10−3	>30000
24		69.9	>0.88 × 10−3	>12600
17		961	3.10 × 10−3	3120
6	Human 2–18 AEFRHDSGYEVHHQKLV	—	—	—
24		—	—	—
17		0.0964 × 105	2.83 × 10−3	293
6	Human 3–18 EFRHDSGYEVHHQKLV	0.027 × 105	4.6 × 10−3	1720	—
24		0.012 × 105	>0.657 × 10−3	> 500	—
17		0.074 × 105	2.41 × 10−3	326	16.3 × 103
6/24/17	Human 4–18 FRHDSGYEVHHQKLV	—	—	—
6	mouse pE3–18 pEFGHDSGFEVRHQKLV	—	—	—
24		—	—	—
17		14.9 × 105	28.6 × 10−3	19

—, no detectable binding (maximal peptide concentration, 1000 nm; maximal 10,000 nm for Aβ_4–18).