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Gamma-toxin is hemolytic to rabbit erythrocytes. 100 μL of gamma-toxin at a 1:1 molar ratio in Tris-Buffered Saline (TBS) at the indicated dose was placed in 0.3 cm diameter wells within 0.4% agar containing 5% rabbit erythrocytes for 4 h and the zone of hemolysis was measured. Data represent area from individual wells.
