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IXN increases DOX-induced apoptosis in MCF-7/ADR cells. Cells were treated with IXN (0–30 μM) alone, DOX (12.5 μM) alone, or in combination for 24 h. (A) Cells were stained by Hoechst 33342, and observed by fluorescence micrograph. Shown is a representative result of three separate experiments. (B) The apoptosis related molecules and survival related signaling molecules were detected by western blotting.
