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. 2017 Jun 30;9(7):208. doi: 10.3390/toxins9070208

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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IXN inhibits the transporting function of ABCB1 in MCF-7/ADR cells. (A) Cells were incubated with DOX (3 μM) alone or in the presence of IXN (7.5–30 μM) for 24 h, then the autofluorescence of intracellular DOX in MCF-7/ADR was detected by flow cytometer moflo XDP (Beckman-Coulter). Histograms show the autofluorescence intensity of DOX. Values are expressed as means ± SD of three separate experiments (p-value relative to control group; * p < 0.05, ** p < 0.01). (B) MCF-7/ADR cells were pretreated with IXN for 6 h, and then with Rho123 for another 2 h. The intracellular Rho123 fluorescence intensity was analyzed by flow cytometry. Histograms show the mean fluorescence of Rho123. Values are expressed as means ± SD of three separate experiments (p-value relative to control group; * p < 0.05, ** p < 0.01). (C) IXN increases the sensitivity of MCF-7/ADR cells to ABCB1 substrate COL. (D) IXN does not enhance the sensitivity of MCF-7/ADR cells to cisplatin, which is not an ABCB1 substrate.

IXN inhibits the transporting function of ABCB1 in MCF-7/ADR cells. (A) Cells were incubated with DOX (3 μM) alone or in the presence of IXN (7.5–30 μM) for 24 h, then the autofluorescence of intracellular DOX in MCF-7/ADR was detected by flow cytometer moflo XDP (Beckman-Coulter). Histograms show the autofluorescence intensity of DOX. Values are expressed as means ± SD of three separate experiments (p-value relative to control group; * p < 0.05, ** p < 0.01). (B) MCF-7/ADR cells were pretreated with IXN for 6 h, and then with Rho123 for another 2 h. The intracellular Rho123 fluorescence intensity was analyzed by flow cytometry. Histograms show the mean fluorescence of Rho123. Values are expressed as means ± SD of three separate experiments (p-value relative to control group; * p < 0.05, ** p < 0.01). (C) IXN increases the sensitivity of MCF-7/ADR cells to ABCB1 substrate COL. (D) IXN does not enhance the sensitivity of MCF-7/ADR cells to cisplatin, which is not an ABCB1 substrate.