Figure 5.

The effect of free rmIL-10 and rmIL-10 conjugated Ag-PVPs on the production of IL-6 by mouse J774 macrophages stimulated with lipopolysaccharide (LPS). Cells (10⁶ cells/mL) were stimulated with LPS (1 μg/mL) and treated with different concentrations of either free rmIL-10 (fresh, S1) or rmIL10-conjugated Ag-PVPs (stored one week at 4 °C). Cell-free culture supernatants were collected at 72 h post-incubation and were used to measure the production of IL-6 by cytokine ELISA. Untreated and LPS-stimulated cells were used as positive controls, and untreated and non-stimulated cells were used as negative controls. * indicates a significant difference (p < 0.01) between the untreated and LPS-stimulated cells versus LPS-stimulated and treated cells as calculated by the unpaired Student’s t-test. The data are presented as means and standard deviations of samples run in duplicate and are representative of three separate experiments.