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. 2017 Jul 24;7(7):193. doi: 10.3390/nano7070193

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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S. aureus ATCC 29213 biofilm (24 h old) on the surfaces of titanium nanotubes with dispersed silver particles formed after topical application of bacteria (A) or using suspension method (B), detected by Alamar Blue assay and Live/Dead BacLight Bacterial Viability kit (alive bacteria). The results are provided as the mean percentage ± standard deviation (SD) of staphylococcal biofilm compared to control biofilm developed on reference sample (Ti6Al4V) considered as 100%. All presented data (except biofilm on TNT20/25 assessed by L/D) were statistically significant (p < 0.05).