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. 2017 Jul 31;8:332. doi: 10.3389/fneur.2017.00332

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Copyright © 2017 Ju, Lee, Kong, Kwon, Choi and Seo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Different fluorescence emission spectra of each tissue in the cochlea. (A) The peaks of intensity are different for five microstructures: Region 1 (red), spiral ganglion; region 2 (green), stria vascularis; region 3 (blue), tectorial membrane; region 4 (yellow), spiral ligament; and region 5 (sky-blue), cochlear bone. (B) Excitation spectra of the five different tissues show that irrespective of their own peaks, most wavelengths exist in the range of 530–610 nm. (C) Although the five regions have different spectra, the combined spectral image stacks of the five microstructures explain why most of the tissues in the cochlea show similar distribution of green autofluorescence.