Table 2.

Primer sequences for quantitative real-time PCR

Gene	Forward sequence	Reverse sequence	Product length (bp)
RNF183	5′-CGAAAAGCTTGAAGGACTGG-3′	5′-TGAAGCAGCTCCAGTGAGAA-3′	166
SELE	5′-GGACACAGCAAATCCCAGTT-3′	5′-CTCCAATAGGGGAATGAGCA-3′	266
ICAM1	5′-GAGATCACCATGGAGCCAAT-3′	5′-CTGACAAGTTGTGGGGGAGT-3′	120
MMP9	5′-TTGACAGCGACAAGAAGTGG-3′	5′-CCCTCAGTGAAGCGGTACAT-3′	129
PLAU	5′-GCCATCCCGGACTATACAGA-3′	5′-ACACAGCATTTTGGTGGTGA-3′	195
CXCR4	5′-TGAGAAGCATGACGGACAAG-3′	5′-GACGCCAACATAGACCACCT-3′	156
IL-8	5′-CTGCGCCAACACAGAAATTAT-3′	5′-CATCTGGCAACCCTACAACAG-3′	214
IL-6	5′-TCAATGAGGAGACTTGCCTGGTGA-3′	5′-TCATCTGCACAGCTCTGGCTTGTT-3′	120
GAPDH	5′-CTCCTCCTGTTCGACAGTCAGC-3′	5′-CCCAATACGACCAAATCCGTT-3′	185

RNF183 ring finger protein 183, SELE selectin E, ICAM1 intercellular adhesion molecule 1, MMP9 matrix metallo peptidase 9, PLAU plasminogen activator urokinase, CXCR4 C-X-C motif chemokine receptor 4, IL-8 interleukin-8, IL-6 interleukin-6, GAPDH glyceraldehyde-3-phosphate dehydrogenase