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. Author manuscript; available in PMC: 2017 Jul 31.
Published in final edited form as: Nat Nanotechnol. 2016 Mar 14;11(7):613–620. doi: 10.1038/nnano.2016.23

Figure 4. Effects of SWNTs and METH on the expression of tyrosine hydroxylase (TH) and the activities of dopamine degradation enzymes (MAO and COMT).

Figure 4

a, Timeline for the biochemical experiments. b,c, Representative (b) and quantitative (c) Western blot assay results, in which the lanes represent the TH or β-actin level in each brain tissue sample as shown above, illustrating that aSWNTs (and iSWNTs to a less extent) significantly attenuated METH-induced increases in TH expression in both the striatum (c, F5,12 = 16.59, p < 0.001) and the PFC (c, F5,12 = 34.61, p < 0.001). d,e, ELISA enzyme activity assays, illustrating that SWNT pretreatment did not alter MAO or COMT activities in either the striatum or the PFC when pre-treated with METH in mice. The error bars indicate the s.e.m. of the mean values from 4 brain samples (from 4 mice) in each group. Each brain sample was replicated three times. ###p < 0.001, compared with the vehicle+saline group (grey bar). **p < 0.01, ***p < 0.001, compared with the vehicle+METH group (black bar). S, saline; V, vehicle.