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Expression levels of several stemness-associated surface markers in PC cell lines. A, CD133, CD24, CD34, CD44 and Pim-3 expression in human PC cell lines were detected by immunoblotting. β-actin was used as internal control. B, PC cells were incubated with fluorochrome-conjugated CD133, CD24, CD44 and ESA antibodies and subjected to flow cytometry analysis. Cells expressing specific markers were drawn in the rectangle gate as well as their percentage in total cells.
