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. 2017 Jun 2;8(9):1530–1541. doi: 10.7150/jca.18628

Figure 2.

Figure 2

CD24+ESA+ PANC-1 cells show stem-cell like phenotypes.A, PANC-1 and L3.6pl cells were simultaneously labeled with PE-conjugated CD24 and APC-conjugated ESA and then analyzed using flow cytometry. B, Colony formation assay was performed using CD24+ESA+ and CD24-ESA- PANC-1 cells and L3.6pl cells. CD24+ESA+ and CD24-ESA- cells were sorted from PANC-1 and L3.6pl respectively using flow cytometry. The colonies were counted 2 weeks after plantation. Data are expressed as the mean ± SD of three independent experiments. *P < 0.05, ***P < 0.001. C, CD24+ESA+ and CD24-ESA- PANC-1 cells and L3.6pl cells were seeded in ultra-low attachment 96-well plate respectively and allowed to grow for 10 days in stem cell culture medium. Spheres (>50 μm) were counted and photographed. Data represent the mean ± SD of three independent experiments. *P < 0.05. D, Tumor number and tumor size in mice 9 weeks after received subcutaneous injection of CD24+ESA+ and CD24-ESA- PANC-1 cells. Data are presented as the mean ± SD; n = 5; **P < 0.01.