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. 2017 Jun 2;8(9):1530–1541. doi: 10.7150/jca.18628

Figure 3.

Figure 3

CD24+ESA+ PC cells exhibit gemcitabine chemoresistance. A and B, CD24+ESA+ PC cells were less sensitive to gemcitabine treatment. The double positive and double negative PANC-1 cells and L3.6pl cells were treated with increasing concentrations of GEM for 72h respectively. Cell viability was determined by CCK-8. Data are expressed as the mean ± SD. The experiments were repeated three times. **P < 0.01, ***P < 0.001. The percentage of CD24+ESA+ PC cells increased by GEM treatment. C and D, Expression levels of CD133, CD24, CD44 and ESA in GEM treated L3.6pl (4 μM) and MIAPaCA-2 (2 μM) analyzed by flow cytometry. Cells expressing specific markers were drawn in the rectangle gate as well as their percentage in total cells.