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. 2017 Jun 2;8(9):1530–1541. doi: 10.7150/jca.18628

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© Ivyspring International Publisher

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.

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Pim-3 induces expression of stemness-associated markers in PC cells. A, Expression level of CD24, CD44 and ESA in PANC-1-Pim-3-sh and MIAPaCA-2-Pim3 analyzed by flow cytometry. Single positive cells (CD24+, CD44+ and ESA+) and their percentage in total cells were drawn in the rectangle gate. CD24+ESA+ cells were shown in the first quadrant as well as their percentage in total cells. B, Expression of other stemness-associated markers were examined in PANC-1-Pim-3-sh, MIAPaCA-2-Pim-3 and their parent cells at protein level by immunoblotting and C, mRNA level by quantitative RT-PCR (mean ± SD; n=3; *P<0.05, **P<0.01, ***P<0.001). D, Expression of Pim-3 and other stemness-associated markers were examined in CD24+ESA+ and CD24-ESA- PANC-1 cells at protein level by immunoblotting and mRNA level by quantitative RT-PCR (mean ± SD; n=3; *P<0.05, **P<0.01, ***P<0.001). E, Colonies formed by PANC-1-Pim-3-sh, MIAPaCA-2-Pim-3 and their parent cells. Data are presented as the mean ± SD. The experiments were repeated three times. **P<0.01.

Pim-3 induces expression of stemness-associated markers in PC cells. A, Expression level of CD24, CD44 and ESA in PANC-1-Pim-3-sh and MIAPaCA-2-Pim3 analyzed by flow cytometry. Single positive cells (CD24+, CD44+ and ESA+) and their percentage in total cells were drawn in the rectangle gate. CD24+ESA+ cells were shown in the first quadrant as well as their percentage in total cells. B, Expression of other stemness-associated markers were examined in PANC-1-Pim-3-sh, MIAPaCA-2-Pim-3 and their parent cells at protein level by immunoblotting and C, mRNA level by quantitative RT-PCR (mean ± SD; n=3; *P<0.05, **P<0.01, ***P<0.001). D, Expression of Pim-3 and other stemness-associated markers were examined in CD24+ESA+ and CD24-ESA- PANC-1 cells at protein level by immunoblotting and mRNA level by quantitative RT-PCR (mean ± SD; n=3; *P<0.05, **P<0.01, ***P<0.001). E, Colonies formed by PANC-1-Pim-3-sh, MIAPaCA-2-Pim-3 and their parent cells. Data are presented as the mean ± SD. The experiments were repeated three times. **P<0.01.