Figure 2.

Statins inhibit the stemness properties of NPC sphere-forming cells. (A, B) NPC sphere-forming cells were cultured in ultra-low attachment plates in the presence of different statins (1 µM) in stem cell culture medium. (A) Photomicrographic images were taken at the end of the 72-h culture period. Original magnification: ×200. (B) Sphere formation was quantified by multiplying the number of tumorspheres and the surface areas of the tumorspheres in each well. (C) The sphere-forming cells were treated with different concentrations of LV for 48 h and subjected to Western blot analysis. LV, lovastatin; AV, atorvastatin; SV, simvastatin.