Figure 4.
Increased β-catenin activation in ESR1-KO livers after CCl4 treatment. (A) Western blot analysis of total and activated β-catenin, phosphorylated nuclear p65; and total and phosphorylated GSK3β proteins at 12, 24 and 48 hr after CCl4 treatment performed using total liver cell extracts of WT and ESR1-KO rat livers. (B) Bar graphs showing densitometric analysis of the active β-catenin western blot. (C) Bar graphs showing densitometric analysis of the phosphorylated GSK3β western blot. * denotes significant difference at P≤0.05 between WT and KO.