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. Author manuscript; available in PMC: 2018 May 26.
Published in final edited form as: ACS Sens. 2017 May 2;2(5):670–678. doi: 10.1021/acssensors.7b00118

Figure 2.

Figure 2

Array results for tobacco-related standards with DNA-reactive metabolites: (A) recolorized ECL data using arrays featuring RuPVP/enzyme/DNA microwells treated with oxygenated solutions of carcinogens B[a]P, NNK, and NNN and negative control toluene in 1% DMSO + 10 mM phosphate buffer pH 7.4 for 45 s at −0.65 V vs Ag/AgCl, with ECL captured by CCD camera after subsequently applying 1.25 V vs Ag/AgCl for 180 s. (B) Calibration plots of % ECL increase over 1% DMSO control vs concentration of standards. ECL intensity increases proportional to DNA damage that disorders ds-DNA and allows coreactant guanines in the DNA better access to RuIII sites of RuPVP.2