Figure 6.

A validation experiment of the candidate protein Gsk-3β and its inhibitor and the network of the 12 candidates. (A) Twenty-four hours after MCAO operation, Longa’s Neurological Severity Score from the model group revealed remarkable ischaemic injury, while DHI, Gsk-3β inhibition and DHI + GSK-3β can observably decrease the scores, indicating improved neurological function in MCAO rats (Table 4, a rank sum test was used); (B) the brains were stained with TTC; (C) the mean infarct volumes in these groups (Table 5, t-test was used); (D,E) validation of the hippocampus proteins from these groups by Western blotting and t-test was used to test the quantitative data. The results indicated that the rats treated with the Gsk-3β inhibitor in combination with DHI showed a lower degree of ischaemic injury (F) Network of the 12 potential candidate proteins by STRING analysis.