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miR-210 downregulates the expression of junction proteins occludin and β-catenin in the brain of rat pups. (A) The diagram shows the 3′ UTRs of rat OCLN and CTNNB1 transcripts are complementary to the seed sequence of rat miR-210. Pups received either miR-210 mimic or its negative control (Neg. Ctrl) via i.c.v. injection; The protein levels of occludin (B) and β-catenin (C) were determined 24 h following HI by western blotting. n = 6. * p < 0.05, miR-210 vs. Neg. Ctrl.
