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. 2017 Jun 27;18(7):1367. doi: 10.3390/ijms18071367

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The miRNAs of targeting Zfp217. (A) The prediction of miRNAs which targeting Zfp217. The brilliant blue ring represents the predicted miRNAs in human (Homo sapiens, hsa). The pink ring represents the predicted miRNAs in mouse (Mus musculus, mmu). The gray blue ring represents the predicted miRNAs in both human and mouse. Venn diagram was output to statistical result; (B) Conservation of the miRNA binding site in the Zfp217-3′ unstranslated (UTR) region. The miRNA seed match region is highlighted in red; (C,D) Two pmirGLO vector constructs, containing either the Zfp217-3′ UTR-wt or the Zfp217-3′ UTR-mut with corresponding miRNA seed region, were transfected into BHK-21 cells either alone or in combination with negative control or each miRNA mimics. Renilla luciferase activity was used to normalize firefly luciferase activity. Data represents means ± SEM (n = 4); and (E,F) The expression levels of Zfp217 was measured by Western blot and qRT-PCR for different treated groups as indicated. The qRT-PCR data represents means ± SEM (n = 3). * p < 0.05, ** p < 0.01 versus control.

The miRNAs of targeting Zfp217. (A) The prediction of miRNAs which targeting Zfp217. The brilliant blue ring represents the predicted miRNAs in human (Homo sapiens, hsa). The pink ring represents the predicted miRNAs in mouse (Mus musculus, mmu). The gray blue ring represents the predicted miRNAs in both human and mouse. Venn diagram was output to statistical result; (B) Conservation of the miRNA binding site in the Zfp217-3′ unstranslated (UTR) region. The miRNA seed match region is highlighted in red; (C,D) Two pmirGLO vector constructs, containing either the Zfp217-3′ UTR-wt or the Zfp217-3′ UTR-mut with corresponding miRNA seed region, were transfected into BHK-21 cells either alone or in combination with negative control or each miRNA mimics. Renilla luciferase activity was used to normalize firefly luciferase activity. Data represents means ± SEM (n = 4); and (E,F) The expression levels of Zfp217 was measured by Western blot and qRT-PCR for different treated groups as indicated. The qRT-PCR data represents means ± SEM (n = 3). * p < 0.05, ** p < 0.01 versus control.