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. 2017 Jun 27;18(7):1369. doi: 10.3390/ijms18071369

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Genomic polymerase chain reaction (PCR) showing Grhl3 deletion in the epidermis of Grhl3^Δ/−/K14-Cre (constitutive Cre expression) and Grhl3^FL/−/IVL–CreERT2 4-hydroxytamoxifen (4-OHT) induced mice. (Left panel) is a positive control. Middle and right panels show deletion at 3 days; 1, 4, 8 and 16 weeks post 4-OHT (as indicated in red) induction of involucrin (IVL)-Cre, demonstrating a similar ratio of deleted cells (Δ) at 1 and 16 weeks. This suggests that deletion of a Grhl3 floxed allele (Grhl3^FL/–) generates long-lived Grhl3 deleted cells (Grhl3^Δ/–) in suprabasal layers of the epidermis (normal tissue renewal is 2–4 weeks).