Figure 3.

Confirmation of predicted cleavage site proteolysis by HDM proteases. Cleavage efficiencies of the predicted sites present at the 21 potential targets (ΔA/A) by Der p 1, Der p 3 and Der p 6 are represented in blue, red and green, respectively. Individual purified phages were either untreated (A) or treated (∆A) with Der p 1, Der p 3 or Der p 6 for 30 min at 37 °C before ELISA on Ni-NTA HisSorb plates. Data presented as mean of two independent experiments performed in duplicate ± standard error of the mean. Significance was calculated compared to negative control using a Mann-Whitney test (p value 0.05).