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(A) SDS-PAGE and Western blot analyses of rIL-23R (10 nM) proteolysis by Der p 1 (2.5 nM), Der p 3 (0.25 nM) and Der p 6 (0.5 nM). Samples were analyzed after different incubation times. kDa: kiloDalton; M: molecular weight marker (Pierce Unstained Protein MW marker, Thermo Fisher Scientific, Rockford, USA); (B) Western blot analysis using a rabbit anti-human IL-23R (residues 62–75) monoclonal primary antibody and a HRP-conjugated monoclonal anti-rabbit IgG as secondary antibody.
