Table 1.
Phage libraries and the corresponding sequences displayed at the surface of phage particles in the libraries X4-X-X4, X4-R/K-X4 and X4-Y/F-X4. P1 residue is represented in bold. (His6: His-tag; GPGG: glycine/proline spacer; X: random residue; R: arginine; K: lysine; Y: tyrosine; F: phenylalanine; pIII: capsid protein III).
| Protease | Library | Displayed Sequence | P1 Residue |
|---|---|---|---|
| Der p 1 | X4-X-X4 | His6-GPGG-X4-X-X4-GGPG-pIII | X |
| Der p 3 | X4-R/K-X4 | His6-GPGG-X4-R/K-X4-GGPG-pIII | R/K |
| Der p 6 | X4-Y/F-X4 | His6-GPGG-X4-Y/F-X4-GGPG-pIII | Y/F |