ClustalW alignment of the C-terminal sequence comparison of plasma membrane Ca2+-ATPase 1 (PMCA1) obtained from different species. Alignment of the sequences of the PMCAs, starting after the last (10th) putative membrane-spanning domain and ending at the last residue. Residues that are completely conserved are marked with an asterisk (*); those that are highly conserved are indicated by colon (:); while similar residues are indicated by a dot (.). “-” represents interval. PMCA1 sequences used in the alignment are as follows: TcPMCA1, Tetranychus cinnabarinus; TuPMCA1, Tetranychus urticae; ApPMCA1, Acythosiphon pisum; and CbPMCA1, Caenorhabditis briggsae. The PMCA2 sequences used in the alignment are as follows: TcPMCA2, Tetranychus cinnabarinus; TuPMCA2, Tetranychus urticae; IsPMCA, Ixodes scapularis; and CbPMCA1, Caenorhabditis briggsae.