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ELISPOT assay of IFN-γ production by PBMCs and PFCs. (A) SPCs among peripheral blood mononuclear cells (PBMCs) and pleural fluid cells (PFCs) stimulated with TB antigens; (B) Comparison of ELISPOT results between PBMCs and PFCs; black columns show a significant increase in log10 fold elevation of SPCs among PFCs compared to PBMCs; (C) Effect of exogenous Gal-9 and lactose in the absence of TB antigens on the number of IFN-γ-producing SPCs. E6-C10, combination of ESAT-6 and CPF-10.
