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. 2017 Jun 29;18(7):1389. doi: 10.3390/ijms18071389

Figure 5.

Figure 5

Skeletal muscle oxidative stress and constitutive markers expression. Immunofluorescence photomicrographs of: gastrocnem us skeletal muscle superoxide dismutase 1 (A–E); catalase (F–L); cyclooxygenase-1 (M–Q); and sirtuin 3 (R–V) expression of: reserpine-induced myalgia rats (A,F,M,R); controls (B,G,N,S); rats treated with reserpine for two months (C,H,O,T); rats treated with reserpine and then with melatonin at the dose of 2.5 mg/kg/day for two months (D,I,P,U); and rats treated with reserpine and then with melatonin at the dose of 5 mg/kg/day for two months (E,L,Q,V). Nuclei were stained with DAPI (blue). Scale Bar: 20 µm. The quantitative measurement of the immunopositivity of both antioxidant enzymes and constitutive molecules of all experimental animals are shown in Figure 6A–D.