Figure 4.
Effect of WK2-16 on LPS-induced cytokine production and cyclooxygenase (COX)-2 expression in THP-1 cells. (A,B) THP-1 cells (5 × 105 cells/0.5 mL) were dispensed onto 24-well plates and were treated with the indicated concentrations of WK2-16 (2, 5, 10 and 20 μM), SAHA (10 μM) or vehicle for 15 min followed by treatment with LPS (50 ng/mL) for 24 h. Cell-free supernatants were then assayed for the levels of: interleukin (IL)-6 (A); and IL-1β (B) by ELISA. The data were represented as the means ± S.D. from three independent experiments. ### p < 0.01 compared with the resting condition, * p < 0.05 and *** p < 0.001 compared with the vehicle. (C) THP-1 cells (106 cells/mL) treated with LPS (50 ng/mL) for 24 h at the indicated concentrations of WK2-16 (2, 5, 10 and 20 μM) or vehicle for 15 min before treatment with LPS. Cell lysates were obtained and analyzed for COX-2 by WB. The data are represented as the means ± S.D. from three independent experiments. * p < 0.05 and *** p < 0.001 compared with the vehicle.