Figure 6.

WK2-16 has no effect on the activation of the MAPK pathway induced by LPS in THP-1 cells. (A) THP-1 cells (1 × 10⁶ cells/mL) were dispensed onto six-well plates and were treated with WK2-16 (2, 5, 10 and 20 μM) or vehicle for 15 min followed by treatment with LPS (50 ng/mL) for 90 min. Cell lysates were obtained and analyzed for p38 phosphorylation by WB. (B) THP-1 cells were treated with WK2-16 (2, 5, 10 and 20 μM) or vehicle for 15 min followed by treatment with LPS (50 ng/mL) for 30 min. Cell lysates were obtained and analyzed for ERK phosphorylation by Western blotting. (C) THP-1 cells were treated with WK2-16 (5, 10, 20 μM) or vehicle for 15 min followed by treatment with LPS (50 ng/mL) for 45 min. Cell lysates were obtained and analyzed for JNK phosphorylation by WB. The data are represented as the means ± S.D. from three independent experiments. # p < 0.05 and ## p < 0.01 compared with the resting condition.