Figure 7.
WK2-16 induces the recruitment of Yin Yang 1 (YY1) and HDAC1 in LPS-stimulated THP-1 cells. THP-1 cells (1.2 × 107 cells/12 mL) were dispensed on 10-cm dishes and were treated with WK2-16 (10 μM), SAHA (10 μM) or vehicle for 15 min followed by treatment with LPS (50 ng/mL) for 2 h. Cell lysates were obtained, and 1 mg of proteins from each dish was subjected to Co-IP with: anti-HDAC1 antibody (IP: HDAC1) (A); or anti-YY1 antibody (IP: YY1) (B) and was analyzed for HDAC1, YY1, ubiquitinated YY1 (uYY1, the super shift of IP: YY1) and acetylated YY1 (acYY1) expression by WB (IB). As a negative control (Ig G), 1 mg of proteins from cell lysates was immunoprecipitated with anti-IgG antibody. In the input lane, 30 μg of proteins of cell lysates was loaded. The data are represented as the means ± S.D. from three independent experiments. # p < 0.05 and ### p < 0.001 compared with the vehicle, * p < 0.05 and ** p < 0.01 compared with WK2-16 treatment.