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Protease activity of Triticain-α derivatives. The enzymatic reaction rates were determined from the initial slope of the progress curves registered in fluorescent protease activity assay utilizing peptide substrate acetyl-(Pro-Leu-Val-Gln)-7-amino-4-methylcoumarin (Ac-PLVQ-AMC). Activity of papain was used as a control. Error bars denote the standard error of triplicate measurements.
