Figure 4.

Expression and purification of soluble Triticain-α using bacterial expression system: (A) Schematic representation of domain structure of Triticain-α construct employed for the expression in E. coli strain Rosetta gami B (DE3); and (B) sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of cellular lysates (“cell”), extracts (“S”) or insoluble fractions (“P”) obtained from Triticain-α transformants with (+) or without (−) IPTG induction; Ttc – purified Triticain-α-GM (the positions of zymogene and mature enzyme are indicated). “M” denotes molecular weight markers.