Figure 8.
Analysis of tumor necrosis factor-α (TNF-α) gene expression assessed using reverse transcription-polymerase chain reaction (RT-PCR) and densitometric analysis of TNF-α/β-actin mRNA ratio in isolated pancreatic acinar cells: (lane 1) acinar cells obtained from control sensory nerves (SN)-intact rats treated with saline, after isolation, acinar cells incubated in cerulein-free solution; (lane 2) acinar cells obtained from SN-intact rats treated with ghrelin (GHRL), after isolation, acinar cells incubated in cerulein-free solution; (lane 3) acinar cells obtained from SN-intact rats treated with saline, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10−8 M; (lane 4) acinar cells obtained from SN-intact rats treated with GHRL, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10−8 M; (lane 5) acinar cells obtained from rats with capsaicin deactivation of SN (CDSN) and treated with saline, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10−8 M; (lane 6) acinar cells obtained from rats with CDSN and treated with GHRL, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10−8 M; (lane 7) acinar cells obtained from rats with CDSN and treated with GHRL, after isolation, acinar cells incubated in cerulein-free solution. NC = negative control. Reference gene: β-actin. a p < 0.05 compared to control acinar cells obtained from rats with intact SN (lane 1); b p < 0.05 compared to acinar cells stimulated with cerulein after isolation from SN-intact rats treated with saline (line 3); c p < 0.05 compared to acinar cells stimulated with cerulein after isolation from SN-intact rats treated with GHRL (lane 4). In each experimental group, there was at least six observations.