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. 2017 Jun 30;18(7):1402. doi: 10.3390/ijms18071402

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of heat shock protein 70 (HSP70) gene expression assessed using RT-PCR and densitometric analysis of HSP70/β-actin mRNA ratio in isolated pancreatic acinar cells: (lane 1) acinar cells obtained from control sensory nerves (SN)-intact rats treated with saline, after isolation, acinar cells incubated in cerulein-free solution; (lane 2) acinar cells obtained from SN-intact rats treated with ghrelin (GHRL), after isolation, acinar cells incubated in cerulein-free solution; (lane 3) acinar cells obtained from SN-intact rats treated with saline, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10⁻⁸ M; (lane 4) acinar cells obtained from SN-intact rats treated with GHRL, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10⁻⁸ M; (lane 5) acinar cells obtained from rats with capsaicin deactivation of SN (CDSN) and treated with saline, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10⁻⁸ M; (lane 6) acinar cells obtained from rats with CDSN and treated with GHRL, after isolation, acinar cells incubated in solution containing cerulein at a concentration of 10⁻⁸ M; (lane 7) acinar cells obtained from rats with CDSN and treated with GHRL, after isolation, acinar cells incubated in cerulein-free solution. NC = negative control. Reference gene: β-actin. ^a p < 0.05 compared to control acinar cells obtained from rats with intact SN (lane 1); ^b p < 0.05 compared to acinar cells stimulated with cerulein after isolation from SN-intact rats treated with saline (line 3); ^c p < 0.05 compared to acinar cells stimulated with cerulein after isolation from rats with CDSN and treated with saline (lane 5); ^d p < 0.05 compared to acinar cells stimulated with cerulein after isolation from SN-intact rats treated with GHRL (lane 4); ^e p < 0.05 compared to acinar cells incubated in cerulein-free solution after isolation from SN-intact rats treated with GHRL (lane 2). In each experimental group, there was at least six observations.