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. 2017 Jul 3;18(7):1424. doi: 10.3390/ijms18071424

Figure 4.

Figure 4

Effect of MAPK on LPS-induced VCAM-1 expression and of chrysin on p38 and JNK activation in bEnd.3 cells. (a) Cells were pretreated with SB202190 (SB, 10 μM), SP600125 (SP, 10 μM), and PD98059 (PD, 10 μM) for 30 min and then co-stimulated with LPS (10 μM) for 8 h. The levels of VCAM-1 mRNA were evaluated by RT-PCR and quantified by densitometry; and (b,c) cells were pretreated with chrysin (10–100 μM) for 30 min and then co-stimulated with LPS (10 μg/mL) for 1 h. Whole cell extracts were prepared and analyzed by Western blot with anti-phospho p38, anti-phospho JNK, p38 MAPK antibody, or JNK antibody. * p < 0.05 vs. control and # p < 0.05 vs. vehicle, n = 3.