Table 2.

Analytical characterization of the recombinant Cu-complexes of LlwtMT, Lltr1MT, and Lltr2MT, obtained from cultures grown with normal aeration (no complexes could be recovered from low aeration conditions).

MT	ICP-AES a	Neutral ESI-MS b	Exp MM c	Theor MM d	Acidic ESI-MS b	Exp MM c	Theor MM d
LlwtMT	4.0 Zn 13.3 Cu	M14-MT M13-MT M12-MT M15-MT	11,058 10,995 10,934 11,122	11,059.3 10,996.7 10,934.2 11,121.8	Cu12-MT	10,934	10,934.2
					Cu8-MT	10,682	10,684.0
					Cu11-MT	10,869	10,871.6
					Cu10-MT	10,808	10,809.1
					Cu13-MT	10,995	10,996.7
					Cu9-MT	10,747	10,746.5
					Cu14-MT	11,061	11,059.3
Lltr1MT	3.5 Zn 5.8 Cu	M8-MT M9-MT M10-MT M7-MT M6-MT	6925 6988 7050 6862 6796	6926.8 6989.3 7051.9 6864.2 6801.7	Cu4-MT	6675	6676.6
					apo-MT	6425	6426.4
					Cu8-MT	6925	6926.8
					Cu5-MT	6738	6739.1
					Cu6-MT	6801	6801.7
					Cu7-MT	6865	6864.2
Lltr2MT	3.2 Zn 6.5 Cu	M8-MT M9-MT M10-MT M7-MT M6-MT	7344 7407 7471 7285 7224	7347.2 7409.8 7472.3 7284.7 7222.1	Cu4-MT	7095	7097.0
					apo-MT	6845	6846.8
					Cu8-MT	7344	7347.2
					Cu5-MT	7161	7159.6
					Cu6-MT	7220	7222.1
					Cu7-MT	7286	7284.7

^a The Zn(II) and Cu(I)-to-peptide ratio calculated from S and Cu content (ICP-AES data); ^b the deduced M-LlMT (M = Zn or Cu) species were calculated from the mass difference between the holo- and the respective apo-peptides. The major species are indicated in bold, and the rest are in decreasing order of ESI-MS peak intensity; ^c experimental molecular masses corresponding to the detected M-LlMT complexes. The corresponding ESI-MS spectra are shown in Figure 5; ^d theoretical molecular masses corresponding to the M-LlMT complexes.