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CD and ESI-MS spectra of Zn/Cd replacement of BgMT: Shown are spectra (at the +7 charge state) corresponding to the Zn(II)/Cd(II) replacement reaction of recombinant (A) Zn-BgwtMT and (B) Zn-BgKNMT, recorded at neutral pH. In both cases, a 5 µM solution of Zn-BgMT was titrated with up to 16–18 equivalents of CdCl2 at neutral pH.
