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. 2017 Jul 8;18(7):1468. doi: 10.3390/ijms18071468

Figure 2.

Figure 2

Phosphorylation of the extra cellular-regulated kinases ERK1/2 upon stimulation with recombinant CXCR6 in glioblastoma cells. (A) T98G and U251MG glioblastoma cells were stimulated with 25 ng/mL recombinant (rec) CXCR6 for 10 or 15 min, respectively, and phosphorylation of ERK1/2 was investigated by Western blot; equal loading was ensured by reprobing of the membranes with antibodies for the non-phosphorylated kinase ERK2. Stimulation with recombinant CXCR6 yielded a clear phosphorylation signal for both cell lines; (B) when CXCL16 expression was reduced in T98G and U251MG cells to 30–40% by CXCL16-specific siRNA (siCXCL16) as proven by qRT-PCR and Western or dot blotting, ERK1/2 phosphorylation after 10 minutes of stimulation with recombinant CXCR6 was clearly diminished in comparison to control siRNA transfections. Examples of n = 3 independent experiments.