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Expression of upregulated miRNAs in R. rickettsii-infected HMECs: HMECs were infected with R. rickettsii for 3 and 24 h, RNA was extracted, and microarray (A) or q-RT-PCR (B-E) were performed to measure the expression of selected miRNAs. The data is normalized to 18S rRNA and relative expression is calculated by ΔΔCT method. The results are presented as the mean (A) or mean ± SE (B–E) of three independent experiments. The asterisks indicate statistically significant change (p < 0.01).
