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. 2017 Jul 9;18(7):1471. doi: 10.3390/ijms18071471

Figure 5.

Figure 5

NOTCH1 regulation by miRNA-200a-3p. HMECs were transfected with miRNA 200a-3p mimic (1 nM) (A and B) or inhibitor (200 nM) (C and D) for 24–48 h using Lipofectamine® RNAiMAX according to the manufacturer’s instructions prior to infection with R. rickettsii for 24 h. Total RNA was isolated from cells lyzed in the Tri-reagent and miRNA 200a-3p (A,C) and NOTCH1 (B,D) expression was measured by q-RT-PCR. The data presented as mean ± SE of three separate experiments. The asterisks (* = p < 0.05, ** = p < 0.01) indicate statistically significant change.