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. 2017 Jul 10;18(7):1474. doi: 10.3390/ijms18071474

Figure 1.

Figure 1

Expression of CD15s in brain endothelial and lung cancer cell lines: (A) Representative ICC images showing CD15s in hCMEC/D3, NCI-H1299, SEBTA-001, A549 and COR-L105. Hoechst blue was used as a nuclear counterstain. Scale bar = 20 µm; (B) Western blot analysis of CD15s of proteins from the cell lines showed highest CD15s expression in NCI-H1299 and SEBTA-001 followed by hCMEC/D3, COR-L105 and A549. Cyclophilin A was used as a protein loading control; (C) representative flow cytometric histograms (Green: control, purple: hCMEC/D3, black: NCI-H1299, red: SEBTA-001, blue COR-L105 and yellow: A549); (D) quantitative flow cytometric analysis of CD15s expression on hCMEC/D3, NCI-H1299, SEBTA-001, COR-L105 and A549. CD15s was overexpressed in NCI-H1299 and SEBTA-001 with less expression in hCMEC/D3, COR-L105 and A549. n = 3, *** p < 0.001 and ** p < 0.01. Data is expressed as ±SE.