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. 2017 Jul 10;18(7):1474. doi: 10.3390/ijms18071474

Figure 3.

Figure 3

(A) CD15s immunoblocking reduced the adhesion of lung cancer cells under static conditions. Confocal images (top panel) showing adhesion of green fluorescently labelled NSCLCs on a brain endothelial cell monolayer (blue) and semi-quantitative analysis of confocal images (lower panel) showed a significant decrease in adhesion ability of NSCLC cells to adhere to hCMEC/D3 cells. n = 3, *** p < 0.001 and ** p < 0.01. Scale bar = 20 µm. Data is expressed as ±SE. We used the same concentration of TNF-α as optimised in a previous study [5] to activate the expression of CD62E on brain endothelial cells. Brain endothelial cells (hCMEC/D3) were exposed to 25 pg/mL of TNF-α for 18 h and levels of CD62E were detected using flow cytometry (Data not shown); (B) blocking with CD15s mAb significantly decreased the adhesion of NSCLC to brain endothelium. Quantitative adhesion assay of human primary and metastatic lung cancer (NSCLC) cells blocked with CD15s mAb (blue bar), non-specific isotype IgM (white bar) to assess the efficiency and specificity of CD15s mAb-blocking efficiency and NSCLC cells on a monolayer of hCMEC/D3 (grey bar) as a negative control. n = 3, *** p < 0.001. Data is expressed as ±SE.